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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene continues to be observed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was identified to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a significant function in marketing mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are described. Two additional splice variants were documented but detailed nucleotide sequence info will not be still out there.
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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide usually includes the epitope regarded by the antibody.
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This manual outlines additional probable optimization of mobile and immunoassay parameters to be sure the best possible success are...
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This manual outlines additional doable optimization of cellular and immunoassay parameters to make certain the very best outcomes are...
Antibodies sure to the blocking peptide now not bind on the epitope about the target protein. By comparing the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is specific.
Concentrate on Data Cyclin E belongs for the highly conserved cyclin family members, whose users are characterized by a extraordinary periodicity in protein abundance from the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins exhibit distinct expression and degradation styles which lead for the temporal coordination of each mitotic party. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S transition. This protein accumulates within the G1-S section boundary and it is degraded as cells development through S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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